Inhibin in immature rat Sertoli cell conditioned medium: A 32 kDa αβ-B dimer
نویسندگان
چکیده
منابع مشابه
Inhibin in immature rat Sertoli cell conditioned medium: a 32 kDa CY./?-B dimer
Conditioned medium of cultured Sertoli cells from 21-day-old rats was used as starting material for the isolation of inhibin. Inhibin activity was monitored by the dose dependent suppression of the folliclestimulating hormone release of cultured rat pituitary cells. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis of the highly purified inhibin preparation revealed a 32 kDa p...
متن کاملShort-term stimulatory effect of Sertoli cell conditioned medium on Leydig cell steroidogenesis is not mediated by inhibin.
Addition of concentrated rat Sertoli cell conditioned medium (rSCCM) to isolated Leydig cells from immature rats stimulated steroid production more than 13-fold within 4 h. LH-stimulated steroidogenesis was not enhanced by addition of rSCCM. The biological activity of the concentrated rSCCM was higher after incubation of Sertoli cells with FSH, whereas FSH alone did not stimulate steroid produc...
متن کاملPachytene spermatocytes in co-culture inhibit rat Sertoli cell synthesis of inhibin B-subunit and inhibin B but not the inhibin -subunit
This study investigates the effects of spermatogenic germ cells on inhibin -subunit and B-subunit expression, and inhibin -subunit and inhibin B production by rat Sertoli cells in vitro. Sertoli cells isolated from 19-day-old rats were cultured for 48 h at 32 C, in the presence or absence of FSH (2·3–2350 mIU/ml), and in the presence of pachytene spermatocytes, round spermatids or cytoplasts of...
متن کاملEstrogen synthesis by immature rat sertoli cells in vitro.
Past research has implicated both the Leydig and Sertoli cells as sources of testicular estrogens. To study this issue further, we investigated the ability of Sertoli cells isolated from the testes of immature (10-day-old) rats to synthesize estrogens in response to FSH in vitro. The aromatase activity of Sertoli cells was examined by comparing simultaneously two different methods: 1. the conve...
متن کاملSchwann cell-conditioned medium inhibits angiogenesis.
Neuroblastomas are biologically heterogeneous tumors that consist of two main cell populations: neuroblastic/ganglionic cells and Schwann cells. The amount of Schwannian stroma strongly impacts prognosis, and favorable outcome is associated with tumors that are Schwannian stroma rich/stroma dominant. At the present time, there is controversy regarding the origin of Schwann cells in neuroblastom...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Endocrinology
سال: 1990
ISSN: 0303-7207
DOI: 10.1016/0303-7207(90)90064-f